Sequence analysis of spontaneous mutations in a shuttle vector gene integrated into mammalian chromosomal DNA.
نویسندگان
چکیده
منابع مشابه
Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector.
In order to study mutagenesis in mammalian cells, stable mouse L-cell lines were established with multiple copies of a lambda phage vector that contains the supF gene of Escherichia coli as a target for mutagenesis. Rescue of viable phage from high molecular weight mouse cell DNA using lambda in vitro packaging extracts was efficient (5 phage per microgram of cell DNA per copy) and yielded a ne...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1987
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.84.10.3354